THE ISOLATION AND SYNTHESIS OF GLUCOSE-l- PHOSPHORIC ACID*

Glucose-l-phosphoric acid was first isolated from minced and washed frog muscle which had been incubated for 3 hour in phosphate buffer containing small amounts of adenylic acid (2). Under these conditions part of the glucose-l-phosphoric acid (l-ester) was converted to hexose-6-phosphoric acid (8ester), so that fractional crystallization of the brucine salts had to be resorted to as a means of separating the two esters. This rather tedious step in the preparation became unnecessary when it was found that the l-ester was the main reaction product in a dialyzed muscle extract incubated with glycogen, inorganic phosphate, and adenylic acid* (3). Glucose-l-phosphoric acid has also been prepared synthetically (1). In the present paper are recorded experimental data concerning the preparation, synthesis, and properties of the l-ester.